DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase
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چکیده
منابع مشابه
DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase
DNA polymerases achieve high-fidelity DNA replication in part by checking the accuracy of each nucleotide that is incorporated and, if a mistake is made, the incorrect nucleotide is removed before further primer extension takes place. In order to proofread, the primer-end must be separated from the template strand and transferred from the polymerase to the exonuclease active center where the ex...
متن کاملRegulation of DNA polymerase exonucleolytic proofreading activity: studies of bacteriophage T4 "antimutator" DNA polymerases.
DNA polymerases replicate DNA with high fidelity lab, which demonstrated that single point mutations in the T4 DNA polymerase gene can increase or decrease because of accurate nucleotide incorporation coupled with exonucleolytic proofreading to remove misinmutation rates by 100-fold or more (Drake et al. 1969). Mutational analysis is a powerful method to probe corporated nucleotides. This state...
متن کاملProperties of bacteriophage T4 mutants defective in DNA polymerase.
G E N E 43 of bacteriophage T4 resides at approximately 320" on the standard map ( STAHL, EDGAR and STEINBERG 1964). Studies of temperatwe-sensitive ( t s ) and amber (am) mutants reveal that gene 43 is the structural gene for the viral DNA polymerase (DE WAARD, PAUL and LEHMAN 1965; WARNER and BARNES 1966; NOSSAL 1969). The T4 DNA polymerase resembles the Escherichia coli DNA polymerase in its...
متن کاملDNA polymerase of bacteriophage T4 is an autogenous translational repressor.
In bacteriophage T4 the protein product of gene 43 (gp43) is a multifunctional DNA polymerase that is essential for replication of the phage genome. The protein harbors DNA-binding, deoxyribonucleotide-binding, DNA-synthesizing (polymerase) and 3'-exonucleolytic (editing) activities as well as a capacity to interact with several other T4-induced replication enzymes. In addition, the T4 gp43 is ...
متن کاملAnalysis of inhibitors of bacteriophage T4 DNA polymerase.
Bacteriophage T4 DNA polymerase was inhibited by butylphenyl nucleotides, aphidicolin and pyrophosphate analogs, but with lower sensitivities than other members of the B family DNA polymerases. The nucleotides N2-(p-n-butylphenyl)dGTP (BuPdGTP) and 2-(p-n-butylanilino)dATP (BuAdATP) inhibited T4 DNA polymerase with competitive Ki values of 0.82 and 0.54 microM with respect to dGTP and dATP, res...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2007
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkm591